When it comes to the tuning of a didgeridoo, there are many factors in determining wether its in tune or not.

Makers have different tuning styles, our instruments are tuned at 22 degree Celsius with bottom end of instrument suspended in the air.

A player with an aggressive approach and tight cheeks and lips will tend to play slightly sharp.

A player with a relaxed approach will tend to play slightly flat.

Then there is wether you play front on or to the side.

All our instruments have been tuned playing front on style.

When tuning our instruments we have a room temperature of 22 degree Celsius, the instrument is played for about 15 min to warm the internal bore, then the final tuning is completed.

The air temperature inside the bore, rather than the instrument itself, is the critical factor here.
Because cold air is denser than warm air, the air molecules provide greater resistance to the sound vibrations and thus lower the pitch.
 
The pitch deviation of larger instruments is wider than that of smaller ones. 
Those with large bores and longer tubing take even longer to change temperature.

We expect a pitch change of about 3 cents for each one degree Celsius change in temperature.
So for every degree minus 22 degree Celsius your instrument will be 3 cents flat, or for every degree above 22 degree Celsius your instrument will be 3 cents sharp.
 

TUNINg                

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Temperature has an important
influence on pitch.
A cold instrument will tend to play flat, 

while one exposed to relatively high temperatures 

will tend to play sharp.

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